TB-500

Thymosin β4 Acetate · also listed as TP-500

The active fragment of Thymosin β4, a naturally occurring actin-sequestering peptide. Studied alongside BPC-157 for its role in cell migration and tissue remodelling.

Sizes

• 10mg / vial — $80 (currently out of stock)

Specifications

• CAS number: 77591-33-4
• Molecular formula: C212H350N56O78S
• Molecular weight: 4963.44 g/mol
• Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr
• Form: Lyophilised powder
• Storage: −20°C, protected from light

What it is

TB-500 is a synthetic version of the active region of Thymosin β4, a protein present in nearly every human cell and in high concentration in wound fluid and blood platelets. Where BPC-157 is studied for local structural repair, TB-500 is studied for cell mobility — how quickly repair cells can travel to where they are needed.

Background

TB-500 is a synthetic fragment corresponding to the active region of thymosin beta-4, a 43-amino-acid protein found in most human cell types. The fragment, not the full protein, is what research suppliers sell.

How it works

Thymosin β4 is the primary actin-sequestering protein in mammalian cells. TB-500 binds actin and regulates how quickly that cytoskeletal scaffolding assembles and disassembles, which is the single mechanism underneath most observed effects: cell migration, blood-vessel formation and reduced fibrotic deposition.

Why researchers order it

Its low molecular weight relative to the full protein gives it wide distribution in circulation rather than staying local to an injection site — the property that makes it the standard research pair to BPC-157 rather than a substitute for it. Also listed by some suppliers as TP-500; same compound.

Studied in

• Actin-binding and cytoskeletal reorganisation models
• Examined in cardiac and dermal repair literature
• Core component of our combination blends

Handling and storage

Add 2ml of bacteriostatic water down the vial wall for 5mg/ml. Swirl to dissolve, do not shake. Store unopened vials at −20°C, protected from light; lyophilised powder is stable for years that way and tolerates shipping at ambient temperature. Solutions prepared for analysis should be held at 2–8°C, protected from light. Repeated freeze–thaw cycles compromise sample integrity.

Stability notes for this sequence

• The sequence contains methionine, which oxidises in solution. Keep reconstituted material out of light and recap the vial promptly.
• Lyophilised powder is stable for years at −20°C in the dark and tolerates days at ambient temperature in transit.

Specification in brief

TB-500 is a 20-residue peptide with the molecular formula C212H350N56O78S and a molecular weight of 4963.44 g/mol, CAS registry number 77591-33-4. The vial is sealed and lot-numbered, holds lyophilised powder, and is kept at −20°C protected from light until it ships.

Sizes and price

TB-500 is sold per vial in one size: 10mg at $80 (currently out of stock). Any quantity can be ordered from a single vial upward; ten vials make a sealed box. Shipping is a flat $10 within the United States and free on orders of $250 or more.

Evidence at a glance

The 3 papers cited below are 2 animal studies and 1 review. None is a controlled study of TB-500 in humans, and this page does not claim otherwise.

Also in Repair & Recovery

• BPC-157 — Body Protection Compound · Pentadecapeptide
• KPV — Lysine-Proline-Valine · α-MSH C-terminal tripeptide

Side by side

• BPC-157 vs TB-500
• TB-500 vs KPV

Further reading

• Repair and recovery peptide comparison
• How to read a certificate of analysis
• Lyophilised peptide storage and stability
• Reconstitution concentration calculator

References

1. Goldstein AL et al. (2012). Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications. Expert Opin Biol Ther. PubMed 22074294 · SummaryReview of thymosin β4 as an actin-sequestering peptide, its role in wound healing and cardiac repair, and early clinical work.
2. Malinda KM et al. (1999). Thymosin beta4 accelerates wound healing. J Invest Dermatol. PubMed 10469335 · SummaryFull-thickness skin wounds in rats: thymosin β4 accelerated closure, re-epithelialisation and collagen deposition.
3. Bock-Marquette I et al. (2004). Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. PubMed 15565145 · SummaryThymosin β4 activated integrin-linked kinase in cardiomyocytes and improved cardiac function after coronary ligation in mice.

For laboratory research use only. Not for human or veterinary use. Nothing on this page is a claim of safety or efficacy, and none of it has been evaluated by the FDA.